منابع مشابه
Preferential integration of marker DNA into the chromosomal fragile site at 3p14: an approach to cloning fragile sites.
Fragile sites are specific regions of chromosomes that are prone to breakage. In cells cultured under conditions that induce fragile site expression, high levels of inter- and intrachromosomal recombination have been observed involving chromosomal bands containing fragile sites. To determine whether expression of specific fragile sites would facilitate preferential integration of exogenous DNA ...
متن کاملA polymorphic DNA marker at the D10S106 locus.
Source and Description: G2—2 E is a single copy 200 bp subfragment of G2 cloned into the EcoRV site of Bluescript KSII(+). Clone G2 was isolated from a library highly enriched for (TTAGGG)n-associated sequences (1). Polymorphisms: TaqI identifies a two allele polymorphism as assessed in 29 unrelated Caucasians. Allele Size No. of Chromosomes Frequency Al 900 bp 31 (0.54) A2 700 bp 27 (0.46) Pst...
متن کاملIsolation of a new marker and conserved sequences close to the DiGeorge syndrome marker HP500 (D22S134).
End fragment cloning from a YAC at the D22S134 locus allowed the isolation of a new probe HD7k. This marker detects hemizygosity in two patients previously shown to be dizygous for D22S134. This positions the distal deletion breakpoint in these patients to the sequences within the YAC, and confirms that HD7k is proximal to D22S134. In a search for coding sequences within the region commonly del...
متن کاملA rapid, non-radioactive screening test for fragile X mutations at the FRAXA and FRAXE loci.
Screening of referrals for the mutations associated with the fragile X syndrome constitutes a significant workload in many genetics laboratories. Since the great majority of these referrals will be negative, there is a need for a rapid and inexpensive screening test. We have developed an assay which allows simultaneous amplification of the triplet repeat sequences at the FRAXA and FRAXE loci by...
متن کاملChromosomal fragile site FRA16D and DNA instability in cancer.
It has been proposed that common aphidicolin-inducible fragile sites, in general, predispose to specific chromosomal breakage associated with deletion, amplification, and/or translocation in certain forms of cancer. Although this appears to be the case for the fragile site FRA3B and may be the case for FRA7G, it is not yet clear whether this association is a general property of this class of fr...
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ژورنال
عنوان ژورنال: Genomics
سال: 1990
ISSN: 0888-7543
DOI: 10.1016/0888-7543(90)90457-6